Ligation sequencing amplicons - dual barcoding (SQK-LSK109 with EXP-NBD104, EXP-NBD114, and EXP-PBC096).Ligation sequencing amplicons - native barcoding (SQK-LSK109 with EXP-NBD104 and EXP-NBD114).Ligation sequencing gDNA - native barcoding (SQK-LSK109 with EXP-NBD104 and EXP-NBD114).The Native Barcoding Kit features: Feature Each barcode adapter also has a cohesive end and this is used as a hook to ligate to the supplied sequencing adapters. Barcoded samples are then pooled together. The library preparation method is similar to the Ligation Sequencing Kit protocol DNA ends are repaired and dA-tailed using the NEBNext End Repair/dA-tailing module, and then a unique dT-tailed barcode adapter is ligated on the dA-tailed template. cDNA – use in combination with Direct cDNA Sequencing Kit.gDNA – use in combination with Ligation Sequencing Kit.
The Native Barcoding Expansion 1-12 is an expansion pack, which provides 12 unique barcodes to enable PCR-free multiplexing of dsDNA samples such as: Multiplexing samples onto one flow cell can reduce the cost per sample for a user. are interested in utilising upstream processes such as size selection or whole genome amplification.īarcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together, making more efficient use of the flow-cell.want to optimise their sequencing experiment for throughput.need a PCR-free method of multiplexing to preserve additional information such as base modifications.wish to multiplex samples to reduce price per sample.
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